1. Process Application in Three Stages of Soybean Peptide Production
1.1 Raw Material Leaching: Coarse Crude Fiber Separation after Defatted Soybean Meal Slurrying (Primary Separation)
Raw material: Low-temperature defatted soybean meal mixed with warm water to prepare feed slurry (solid content: 6%~12%, pH 7.5~8.5 for alkaline extraction of soluble protein).
- Function: Separate coarse soybean dregs containing crude fiber and soybean hull impurities; overflow clear liquid is crude soybean protein solution as feedstock for subsequent enzymolysis. Discharged cake moisture content ranges from 68% to 72%, available for secondary water extraction to recover residual peptides for higher product yield.
- Advantage: Continuous feeding, free of filter cloth clogging and adaptable to fluctuating feed concentration.
1.2 Post-enzymolysis Primary Separation: Removal of Undigested Macromolecular Protein & Fine Residues (Core Working Section)
After enzymolysis at 50~60℃ for 2~4 h with neutral/alkaline protease followed by enzyme deactivation, the hydrolysate is fed into
decanter centrifuge for key purification of soybean peptides:
- Liquid overflow: Rich in target small-molecule soybean peptides (300~3000 Da), directly delivered to activated carbon decolorization, concentration and spray drying to produce finished soybean peptide powder.
- Solid cake discharged via spiral conveyor: Denatured undigested macromolecular protein and fine soybean residues, which can be recycled for secondary enzymolysis or processed into feed-grade protein powder as by-product.
1.3 Isoelectric Precipitation & Acid Precipitation for Deep Purification
The clarified enzymolysis liquid is pH-adjusted to 4.2~4.5 (isoelectric point of soybean protein), inducing flocculation and precipitation of macromolecular impurity protein, then further separated by decanter:
- Overflow clear liquid: High-purity aqueous soybean peptide solution with low ash content and reduced bitter taste, improving finished peptide purity by 8%~12%.
- Discharged solid: Acid-precipitated crude protein residue for by-product recovery.
Defects of traditional process: Plate-and-frame filtration requires filter aids such as diatomite, intermittent manual cake discharge, resulting in over 10% peptide loss. Decanter centrifuge needs no filter aid, runs under fully sealed continuous condition and increases peptide recovery rate by 5%~9%.
2. Food-grade Decanter Selection & Standard Operating Parameters
2.1 Model Selection (All contact parts in SUS304/SUS316L food-grade stainless steel, spiral blades with tungsten carbide overlay for abrasion resistance)
| Capacity |
Recommended Model |
Throughput |
| Small scale: 500~1500 L/h |
LW350 / LW430 food-specialized type |
0.5~1.5 m³/h |
| Medium scale: 2~5 m³/h |
LW450 / LW530 |
2~5 m³/h |
| Large scale: 5~12 m³/h |
LW650 / LW720 |
5~12 m³/h |
2.2 Operating Process Parameters for Soybean Peptide Working Condition
- Separation factor G: 2200~3000 G (Medium-high G-force balances clarification efficiency and cake dewatering against relatively high viscosity of peptide solution)
- Bowl rotating speed: 2200~2900 rpm; differential speed: 5~12 rpm (Lower differential speed improves solid dewatering with slightly turbid overflow; medium differential speed is preferred for peptide production)
- Feed solid content: 3%~15%, feed temperature: 45~60℃ (inherent temperature of raw enzymolysis liquid)
- Discharge index: Overflow turbidity ≤15 NTU; discharged cake moisture: 65%~72%
3. Exclusive Advantages for Soybean Peptide Production
- Fully sealed continuous production: Prevent oxidation and browning of soybean peptides by avoiding air exposure, optimizing finished product color and flavor while mitigating bitter taste.
- Excellent adaptability to feed fluctuation: Tolerant to variable particle size ranging from 0.005 mm fine protein flocs to 0.5 mm coarse soybean residues and fluctuating solid content; disc separator is prone to clogging and filter equipment suffers frequent filter cloth blinding.
- Compatible with CIP (Clean-in-place) sanitization: Sanitary structural design for in-situ cleaning, compliant with GMP food regulation and eliminating hidden risks of residual protein spoilage.
- Value-added by-product utilization: Separated crude protein residues are collected and processed into feed-grade soybean protein powder to maximize overall economic benefit.
4. Downstream Matching Process Flow
Decanter clarified liquid → Activated carbon decolorization & debittering → Flash deodorization → Vacuum concentration → High-pressure homogenization → Spray drying → Finished low-molecular-weight soybean peptide powder with superior water solubility and high bioavailability.
5. Troubleshooting & Process Optimization
- Turbid overflow with residual fine solids entrained: Slightly raise bowl rotating speed, reduce differential speed and cut down feed flow rate.
- Excessively wet cake (moisture>75%): Lower differential speed, adjust to shallow pond depth to extend dewatering residence time in conical bowl section.
- High-viscosity hydrolysate: Preheat feed liquid to 55~60℃ to reduce viscosity and enhance separation performance.